Advanced genebank management of genetic resources of European wild apple, Malus sylvestris, using genome-wide SNP array data

Abstract The Netherlands’ field genebank collection of European wild apple ( Malus sylvestris ), consisting 115 accessions, was studied in order to determine whether duplicates and mistakes had been introduced, develop a strategy optimize the planting design as seed orchard. We used 20K Infinium single nucleotide polymorphism (SNP) array, developed M. domestica , for first time genotyping . could readily detect clonal copies unexpected duplicates. Thirty-two accessions (29%) showed close genetic relationship (parent-child, full-sib, or half-sib) another accession, which reflects small effective population size situ populations. Traces introgression from were only found 7 individuals. This indicates that pollination preferentially took place among trees. conclude can be considered mainly pure accessions. results imply it should managed one unit when production. A bias allele frequencies seeds may prevented by not harvesting all with others discuss value using SNP array elaborate resources more depth, including phasing markers subset step towards management at level haplotypes.